, , . , 㳿 , , , . , , . 33 (, ).
(. 33). , , : .
, , , (Cyrus Levinthal). ~10 , 100 10100 . ~1013 , , 1080 , . (. 33) : , , . , .
. , . . , ( ), , 㳿.
, 㳿, . , . (. 34) , , .
. , , . ³, . 㳿, . , , ( , 䳿).
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. 33. 㳿 (G) (q1, q2), .
. 34. (. . 33).
, , . in vitro , : - . , :
, , . - 㳿 : , , 㳿.
. , : , , .
, in vivo , ~100 / . , , , . ( .chaperon , , ).
, , . , . , . hsp (heat shock proteins). hsp , hsp 30 40 ( ).
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hsp70
. , ~100 Å, ~15 Å ~30 . , . hsp70 ( ) , . -, 䳺 (. 35), -.
. 35. - hsp70 ( DnaK) () 7 (1DKZ). , .
: . , hsp70.
ADP. , / . ó ( - hsp70 - hsp40) - . - ADP (. 36).
- . , : ( ) hsp70 . , , hsp70. : - , , . , . (, , . ), , hsp70, .
hsp70D , , : . hsp70 ( ), hsp90.
. 36. - hsp70.
hsp70 . . HSF (Heat Shock Factor) hsp40, hsp70, hsp90, . ϳ , , hsp HSF , hsp, . ϳ hsp HSF.
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: hsp70 ( ) hsp90 - 23, - .
, hsp60, ᒺ . ( ) , hsp60 ( GroEL). ~45 Å , (. 37), , 7 hsp10 ( GroES).
. 37. GroEL (1WE3) . hsp60 ,7 hsp10 .
( ) , , , / .
, , (. 38). ( , ) ADP : 1) hsp10 ( ); 2) . (ADP ) . hsp10 ( ) ( ) .
. 38. hsp60 (1WE3): ADP hsp10 () ().
, ADP ( ), : ( . 37).
. 39. . ʳ hsp60 ( . 39, ) , : , . : , ( ) . ~20 , , ( hsp60) ADP. ADP : , . 20 , . , .
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. 39. . : , . ( . 37, 38), hsp10.
Ͳ
1. ? ? 䳿 ?
2. ? , .
3. -? ?
4. - ?
5. ? ? .
6. ? ?
7. ?
8. .
9. ?
10. EF1 EF2.
11. GTP ?
12. ?
13. - ?
14. ? ?
15. ?
16. ?
17. - .
18. 䳿 .
19. ?
20. 䳿 . 㳺 ? ; ?
21. ?
22. hsp70.
23. .
˲
, .., , .., , .. -. .: , 1984.
, .., , .. . . .: , 2005
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