, 15 , . . , : NA = n sin α. n ~ 1.5, sin α ≤1, NA ≤ 1.5. NA = 1.45, .. . , , ! 4p, 4-.
, (. 3). , , . , PSF ( 4-7 !), .. (. . 5.12).
. 5.12 4- . (LEICA Microsystems).
4- :
) , .
) , .
) .
.
.5.13 PSF 4-
( ).
STED
, STED stimulation emission depletion, .. () . . 4- (S.Hell), : Diffraction resolution limit broken!.
, . STED 2 : 1) () ; 2) , , (.5). ( ) .
. 5.14 STED- SP5 (Leica
Microsystems).
() , PSF , .. . 4- , 90-100 .
|
|
. - . . 2002 Leica (AOBS), . , , , . Carl Zeiss LSM 510 META , 32- . 2004 Zeiss LSM 5 Live, 20 . Olympus , , , FRAP. Nikon . , Leica 2007 . STED - . , .
12