| 15 | 30 | 40 | 45 | 50 | |
| 7% | |||||
| 30% | 3,5 | 7 | 9,3 | 10,5 | 11,7 |
| 10 | 1,5 | 3 | 4 | 4,5 | 5 |
| 15 | 30 | 40 | 45 | 50 | |
| 10% | 133 | 266 | 355 | 400 | 443 |
| 20 | 15 | 30 | 40 | 45 | 50 |
| 8% | |||||
| 30% | 4 | 8 | 10,7 | 12 | 13,3 |
| 10 | 1,5 | 3 | 4 | 4,5 | 5 |
| 15 | 30 | 40 | 45 | 50 | |
| 10% | 133 | 266 | 355 | 400 | 443 |
| 20 | 15 | 30 | 40 | 45 | 50 |
| 10% | |||||
| 30% | 5 | 10 | 13,3 | 15 | 16,7 |
| 10 | 1,5 | 3 | 4 | 4,5 | 5 |
| 15 | 30 | 40 | 45 | 50 | |
| 10% | 133 | 266 | 355 | 400 | 443 |
| 20 | 15 | 30 | 40 | 45 | 50 |
30%
| 100 | 200 | |
| 29 | 58 | |
| 1 | 2 |
10
| 1 | 0,5 | |
| 108 | 54 | |
| 55 | 27,5 | |
| 0,5 =8 ( ) | 40 (9,3 ) | 20 (4,65 ) |
10
| 1 | 0,5 | |
| 48,4 | 24,2 | |
| 11,2 | 5,6 | |
| 0,5 =8 | 20 | 10 |
| 7,8 |
0,09% AgNO3
| AgNO3 | 1,8 |
| 2 | 2000 |
-
| 40% | 15 |
| NaOH | 15 |
| 2 | 500 |
4
| II | |
| (N=6) | |
| Bal I | TGG|CCA |
| Bam HI | G|GATCC |
| Bcl I | T|GATCA |
| Bgl II | A|GATCT |
| Eco RI | G|AATTC |
| Hin dIII | A|AGCTT |
| Hpa I | GTT|AAC |
| Kpn I | GGTAC|C |
| Pst I | CTGCA|G |
| Pvu II | CAG|CTG |
| Sma I | CCC|GGG |
| Sac I | GAGCT|C |
| Sac II | CCGC|GG |
| Sal I | G|TCGAC |
| Xba I | T|CTAGA |
| Xho I | CTC|GAG |
| (N=6) | |
| Acc I | GT|(A/C)(G/T)AC |
| Ava I | C|PyCGPuG |
| Hae I | (A/T)GG|CC(T/A) |
| Hae II | PuGCGC|Py |
| Hgi AI | G(T/A)GC(T/A)|C |
| Hin dII | GTPy|PuAC |
| (N=5) | |
| Asu I | G|GNCC |
| Ava II | G|G(A/T)CC |
| Eco RII | |CC(A/T)GG |
| Hin fI | G|ANTC |
| (N=4) | |
| Alu I | AG|CT |
| Fnu DII | CG|CG |
| Hae III | GG|CC |
| Hha I | GCG|C |
| Hpa II | C|CGG |
| Mbo I | |GATC |
| Taq I | T|CGA |
| (N=4) | |
| Dpn I | CmATC |
| Msp I | C(mC/C)GG |
5
, SSCP-
SSCP-
|
|
|
| NaOH 5M | 10 |
| 0,5 | 10 |
| 2 | 90 |
-. , 30 .
SSCP ( 50 )
| 10% | 8% | ||
| 30% 29:1 | 16,7 | 30% 29:1,3 | 13,3 |
| 10 | 2,5 | 10 | 2,5 |
| 50% | 5 | 50% | 5 |
| 50 | 50 | ||
| 10% | 443 | 10% | 443 |
| 2 | 50 | 2 | 50 |
6
| U | C | A | G | ||
| U | Phe(F) Phe(F) Leu(L) Leu(L) | Ser(S) Ser(S) Ser(S) Ser(S) | Tyr(Y) Tyr(Y) Term Term | Cys(C) Cys(C) Term Trp(W) | U C A G |
| C | Leu(L) Leu(L) Leu(L) Leu(L) | Pro(P) Pro(P) Pro(P) Pro(P) | His(H) His(H) Gln(Q) Gln(Q) | Arg(R) Arg(R) Arg(R) Arg(R) | U C A G |
| A | Ile(I) Ile(I) Ile(I) Met(M) | Thr(T) Thr(T) Thr(T) Thr(T) | Asn(N) Asn(N) Lys(K) Lys(K) | Ser(S) Ser(S) Arg(R) Arg(R) | U C A G |
| G | Val(V) Val(V) Val(V) Val(V) | Ala(A) Ala(A) Ala(A) Ala(A) | Asp(D) Asp(D) Glu(E) Glu(E) | Gly(G) Gly(G) Gly(G) Gly(G) | U C A G |
7
| - | ||
| GenBank | www.ncbi.nlm.nih.gov/genebank | |
| GeneCards | http://www.genecards.org/ | |
| PubMed | www.ncbi.nlm.nih.gov/pubmed | |
| Entrez Protein | www.ncbi.nlm.nih.gov/protein | |
| SWISS-PROT | web.expasy.org/groups/swissprot | |
| InterPro | www.ebi.ac.uk | |
| OMIM | www.ncbi.nlm.nih.gov/omim/ | |
| Pfam | www.pfam.sanger.ac.uk | |
| PDB | www.rcsb.org/pdb | |
| KEGG | www.kegg.jp |
[, 2012].
- :
www.pubmed.gov
www.ncbi.nlm.nih.gov/pmc
www.sciencedirect.com
www.scopus.com
www.elibrary.ru
http://scholar.google.ru/schhp?hl=ru
www.molbiol.ru
