, , . Q10, . = 55 / 37.
, , , , , . 1.
. 1
, , . . , . . , , 1. , . 2.
. 2
, . .
. , (, ). , , , . M r = 100 c M r = 100000. 1 /. :
(S) = 1×105 /, (E) = 1×108 /
1000 . . .
, . , , .
, , . . 3.
. 3
. - S E, . , P. .
|
|
k 1 k 2
S + E S .... E E + P
k 1
. S, -.
( .)
- :
v = = k2c (S×××E) (1)
= k1c (S) c (E) - k-1c (S×××E) - k2c (S×××E) (2)
() , . c (E)
c (E) = co (E) - c (S×××E) (3)
:
= 0
k1c (S) c (E) - k-1c (S×××E) - k2c (S×××E) = 0 (4)
(3) (4)
k1 c(S)[ co (E) - c (S×××E)] - k-1c (S×××E) - k2c (S×××E) = 0 (5)
(5) - S×××E:
k 1,
(6)
, , KM:
(7)
. 1:
(8)
. 8 -. . k 2 k 1 k 1 - . :
, c (S)>> K, K , c (S) . 8 ; :
v = k 2 c o(E) (9)
. , .
, c (S)<< K, :
v =
, ( I . 4) ( III).
|
. 4
- :
(10)
, .
, . 10 , (. 4):
, K M = c '(S)
. 10 . . 10:
|
|
- 1/ v 1/ c (S) - (. 5). , 1/ v , . , 1/ K M. , 1/ v max 1/ K M, .
. 5
, . -. , v S . , ( , . . 6), . . S .
. 6
, , . . .
() I , S. - , .
|
|
|
|
E S
, . - (1/ v max), (. . 7). , . . , , , E S, .
. 7. () ()
, . . . . , . , - . , . . , , - , .
, , , . .
? . . E S-, . , , E I. (Cd2+, Ag+, Hg2+, Tl+ ), , . .
|
|
. - :
. 8. , .
, , . , .
, -.
, - , , .