, , , . , , :
,
h , c , l - n - .
, (.. ), (.. ). , . , . , -, . (, ). ( ). , . . ; , (, ). (. . 3.5). , .
. 3.5 , (1) (2). (3). . .
, . :
,
1 , E2 , .
. , . . . . , , . , , , . , , . . .
|
|
. , . I0 l C, I --:
I = I0×10 elC, elC elC,
e (), 11, 1 1 . , A (). l = 1 , Dl , l , . , e∙. , , e , , . (, , ) .
1.
2. :
3. ,
4. :
5. ,
6.
7.
1. Lowry O.H., Rosebrough N.J., Farr A.L., Randall A.L., Protein measurement with the Folin phenol reagent, J. Biol. Chem., 1951, 193, 265-275
2. Bradford ., A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding, Anal. Biochem., 1976, 72, 248 254
3. .., , , ., 1987
4. ., ., ., ., , . 1, , ., 1993
5. .., : , . .., , ., 1996
6. .., .., , , ., 1998
7. ., .-., , , ., 2000