1. . File ( 1), New ( 2), New protocol ( 3):
, FS SS.
2. Navios Gallios . (FL1 FITC, FL2 , FL3 ECD, FL4 5), , ( FL5 FL8).
Cytometer ( 1) Cytometer Controls.. ( 2) |
Cytometer Control :
, Parameters .
Parameters | Parameters FL5-FL8 |
. :
, . , Yes , - - , No . , , Yes . :
( File name ), Acquisition Protocol , , 4color-setup. File name Save .
, 4color-setup, FITC , ECD 5.
3. , . .
FL1, FL2, FL3 FL4.
.
, ( ) - Histogram Plot Properties .
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. , , Parameter , ( 1), FL1 FITC ( 2). ( 3).
, , X FL1, Y , .
, FL2, FL3 FL4.
, Ctrl D . . , . |
, 4 , X FL1, Y , .
FL1 FL2, FL3, FL4.
( ). | |
Histogram Plot Properties . ( 1), Format Plot ( 2). |
Format Plot Histogram Plot Properties .
Parameter , ( 1), FL2 ( 2). ( 3).
4 5, FL1 FL3 FL4, . :
, (, , ) |
, , , . : ( ), , , , , .
, , :
Window , Tile Special . |
Tile Special
: - , , . , FL4, . , FL4, | |
. . |
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, :
, .
. . FL1, FL2, FL3 FL4 2, 3, 4 5, ( ).
( 100 ) .
, (, FL1) .
, Single Linear Region |
( FL1), ,
, 100 FL1, . :
, . -, ( ):
, ( , , , , !!!)
( ), . , B .
FL2, FL3 FL4, .
( , 100 X):
FS SS.
, A . ? 10 . , . .
?
:
. , , ,
( ), , , , Region Properties .
Autogating Eliptical None None , .
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, , . , , , , . , . , . , A. ( FL1, FL2, FL3 FL4) .
( FL1), Histogram Plot Properties , . , , Format Plot .
Gate, ( 1) ( A ( 2( |
.
( ). , , A. |
, FL2, FL3 FL4.
Histogram Plot Properties , .
1. , , , ( Navios Gallios 100 000 300 Cytometer Control ), , , . , , , . ( ) A. , . ? , , A FL1, FL2, FL3 FL4, Histogram Plot Properties Stop and Save ( 1), Use Stop Condition ( 2).
, , Maximum Events , ( 5000).
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.
( ). , , , (5000) - , LMD. |
2. , (. ). , , B , .
Histogram Plot Properties , Data Source Show % on plot ( 0,0% ) Apply % on plot to all plot ( 0,0% , ).
0,0% ( )
.
Save Protocol .