, -- : . , , (. 4.6).
. 4.6. HeLa Affinity gel Anti-Flag M2, Anti-Flag M2 () IgG anti cKRS DN ()
: 1 ;
2, 5, 8 , pc5FLAG/TUSC4 pSG5;
3, 6, 9 , pc5FLAG/TUSC4 pCMV6-XL5/KRS;
4, 7, 10 , pc5FLAG pCMV6-XL5/KRS.
, , pCMV6-XL5/KRS (. 4.6, 6 7), (. 4.6, 1) , KRS (. 4.6, 5). . , , NPRL2 (. 4.6, 8, 9 10). , -- 䳺 NPRL2.
䳿 43, MARS, NPRL2
pc5FLAG/TUSC4 pCMV6-XL5/43 , , .
. 4.6. HeLa Affinity gel Anti-Flag M2, Anti-Flag M2 () IgG anti p43 ()
: 1 ;
2, 5, 8 , pc5FLAG/TUSC4 pSG5;
3, 6, 9 , pc5FLAG/TUSC4 pCMV6-XL5/43;
4, 7, 10 , pc5FLAG pCMV6-XL5/43.
, (. 4.7, 6 7) (. 4.7, 1) , 43 (. 4.7, 5). , , MARS, (. 4.7, 8, 9 10).
' mTOR- .
1. HeLa , -- (pCMV6-XL5/MRS), -- (pCMV6-XL5/QRS), -- (pCMV6-XL5/KRS), -- (pCMV6-XL5/DRS), 43 (pCMV6-XL5/p43), NPRL2 (pc5FLAG/TUSC4) .
2. , -, -, -, -- 43 NPRL2 in vivo
3. , -- 䳺 Affinity gel Anti-Flag M2 䳿 NPRL2 .