5000 , . 16- . 5-10 , 1-2 . ʳ , 2-3 . , [2]. , :
1. UAS upstream ;
2. ;
3. I [7].
Upstream (10-30 ..) , 50-100 .. upstream . UAS , . , upstream , UAS downstream. Upstream - , . ֳ, 20% dA: dT , UAS , 䳿 - .
-, . . 40-120 .. upstream , , . - - (BP (TATA binding proteins), - II [7]. , - BP, - - .
. , upstream -, . , , [5, 7].
, . upstream -, UAS. upstream UAS, , UAS - [5].
S. cerevisiae , . ³ , 䳿 [3]. , , 200 100 , - 30 , (. 1).
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. 1.
S. cerevisiae
ֳ , (UASCu), , 32 34 .. - (CITF). . CITF, (ACE1) , , UASCu . CITF N- . UASCu , , , . ֳ , - CITF , [3].
- (Pol II) .
in vitro - , 12 , TBP, TFIIE,TFIIB, TFIIF TFIIH [6]. TBP -, TFII - , TFIIF 䳺 Pol II - , TFIIH - Pol II TFIIE , . , TFII-, TBP - in vitro. TBP- (TAFs (TBP associating factors) Pol II Srb , , , , in vitro. in vitro, [5, 6]. , , , , TBP TFIIB , .
- (TBP) , - core- 180 , 80% . - core- TBP , N- . , core- T- [5, 6].
ore- , . α- β- . - . TBP - .
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, TBP Pol II, -. TBP (SL1, TFIID, TFIIB), -. , TBP in vivo, -.
TBP Pol II- TAFs , TFIID . TFIID in vitro, - -. TAFs . , , TBP- . , TAFs , , - [6].
Pol II TBP TFIID, , TBP, , in vitro TAFs TFIID.
TFII , . TFII in vivo Pol II , Pol II . TFII TBP in vivo.
TFII Pol II , . S cerevisiae - . In vivo, TFII Pol II (Rpb1) , , downstream. , Pol II Rpb 9 upstream [6, 7].
TFII Pol II , . - Pol II. . - Pol II . In vitro Pol II . , Pol II .