8. Incubate .
9. Set Up.
10. Analise. , . , Record Data
11. (Test Tube Washer).
12. . Record Data.
13. , Graph
33. .
. 2.
2.
Experiment Insulin and diabetes Part II. . , , , (, , ), .
:
1. (Saline) . . .
2. (Alloxan), - , . . .
3. (Insulin) , . 3- () 4- () .
4. (Obtain reagents) . , .
5. (Deionized wather), (Barium hydroxide) (Heparin).
6. Mix .
7. Centrifuge .
8. Remuve Pellet .
9. (Enzyme Color Reagent)
10. Incubate .
11. Set Up.
12. Graph Glucose Standard .
13. Analise. , . . . , Record Data
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14. (Test Tube Washer).
15. ( 13). Record Data.
16. .
7.
1. , .
( ) : , .
, . : , .
(Pf) (Pb = 70 mm Hg), (Po = 25 mm Hg), (Pi = 5 mm Hg), :
Pf = Pb - (Po + Pi)
20% , .
: , .
(. 1) , , . (Urine Volume), () (Glomerular Pressure) .. (Glomerular. Filtr. Rate)
, ( , - .). , , (Data Set). (Combined)
:
1. : 0,50; 0, 45; 90 mm Hg; 1200 mosm. Start , .
. 34. .
2. , Record Data.
3. Refil.
4. Afferent Add data set
5. , (+) (-) Start. , (Refil).
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6. Pressure Add data set
7. . .
8. Combined Add data set
9. , . .
10. (. 1) .
2. .
, . -- . - . I, - II. , ..
:
- Na, Cl,
-
-
( ) , . , , .
:
-
-
- .
, , , .
(Glucose carriers).
, , , ; , . , , , .
, , , . , .
. 35. .
:
1. Simulating Urine Formation ( ).
2. : 300 mosm, (carriers glucose) = 0.
3. Gradient Data sets
4. Start , .
5. , Record Data.
6. Dispense.
7. 3-6.
8. Glucose Data sets,
9. (+) (-) Add carriers.
10. Start , . (Dispense).
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11. 9-10 .
12. Hormone Data sets.
13. (Aldosterone) . .
14. (ADH) . .
15. (. .