, , . , . , , . . , - , . , .
.. , .
, 450.
. 5.6. , LEICA TCS SL.
- , . , , . LEICA TCS SP5, SPE, SL, LSM 510 META. FISH Fluorescence in-situ hubridisation, .. .
, , , . , . , , .
. (.. ). , .
.. , .. - . , . , ++ FURA FLUO, .
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FRAP
Fluorescence Recovery After Photobleaching ( ). . .
. 5.7. FRAP, FLIP, (LeicaMicrosystems).
. (. 5.7, 3). , , FLAP - Fluorescence Localization After Photobleaching ( ), FLIP - Fluorescence Loss In Photobleaching ( ), (. . 5.7).
, (. 5.8). (, ) ,
t1/2, :
D = 0.88*w2 /(4 t1/2)
w . , .. .
. 5.8. FRAP. ROI1- , ROI2- (LEICA TCS SL)
FRET
Főrster (Fluorescence) Resonance Energy Transfer - Ը () . , . , ( ). , . , , , . , .
FRET r :
E ~ 1/ (1 + (r/R)6)
R (~ 3 ).
. 5.9 FRET.
FRET: Acceptor photobleaching, Donor photobleaching, Ratio imaging, Sensitized emission, , .